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Zaborowska, Michalina; Matyszewska, Dorota; Bilewicz, Renata, 2024, "Research data for article: Model lipid raft membranes for embedding integral membrane proteins. Reconstitution of HMG-CoA reductase and its inhibition by statins", https://doi.org/10.58132/GIRCLB, Dane Badawcze UW, V1
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In this study, we present the reconstitution of HMG-CoA reductase in phospholipid liposomes composed of DOPC:Chol:SM at a 1:1:1 molar ratio. The liposomes were characterized by DLS method and visualized by fluorescence microscopy. The liposomes and proteoliposomes were spread at the air-water interface to form layers using Langmuir technique. The stability of the layers in time was investigated using Langmuir technique and the morphology was observed by Brewster angle microscopy. The activity of the embedded protein was measured using UV-VIs spectrophotometry.
model biological membranes, protein reconstitution, Langmuir technique
M. Zaborowska, D. Matyszewska, R. Bilewicz, Model Lipid Raft Membranes for Embedding Integral Membrane Proteins: Reconstitution of HMG-CoA Reductase and Its Inhibition by Statins, Langmuir 2022, 38, 13888−13897. https://doi.org/10.1021/acs.langmuir.2c02115 https://doi.org/10.1021/acs.langmuir.2c02115 doi: 10.1021/acs.langmuir.2c02115
CC BY - Creative Commons Attribution 4.0
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