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Brzóska-Wójtowicz, Edyta, 2025, "Wpływ nadekspresji mikroRNA-126 na zmiany w transkryptomie ludzkich mioblastów", https://doi.org/10.58132/2W1FF5, Dane Badawcze UW, V1
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Projekt dotyczy wpływu nadekspresji mikroRNA-126-3p lub mikroRNA-5p na zmiany w transkryptomie ludzkichch mioblastów. Komórki transfekowano 25nM mimikowym mikroRNA. Po 48h wyizolowano całkowite RNA i wykonano analizę NGS (Next Generation Sequencing Core Facility, Centrum Nowych Technologii). Dane z wysokoprzepustowego sekwencjonowania występują w postaci:
„rawCounts” - pliku z surowymi danymi (.txt)
„comparsion” - list zidentyfikowanych transkryptów (.xlsx)
dla poszczególnych wariantów eksperymentu : 3p1, 3p2,3p3 – transfekcja mikro RNA-126-3p; 5p1,5p2,5p3 – transfekcja mikroRNA-126-5p, K1,K2,K3- kontrola.
The project focuses on the impact of microRNA-126-3p or microRNA-126-5p overexpression on changes in the human myoblasts transcriptome. The cells were transfected with 25nM mimic microRNA. After 48 hours, total RNA was isolated and NGS analysis was performed (Next Generation Sequencing Core Facility, Center for New Technologies). Data from high-throughput sequencing are available as:
„rawCounts” – non-normalized reads associated with each reference transcript (.txt)
„comparsion” - results of expression comparison for each detected transcript (.xlsx)
for individual experimental variants: 3p1, 3p2, 3p3 – transfection with microRNA-126-3p; 5p1, 5p2, 5p3 – transfection with microRNA-126-5p, K1, K2, K3 – control.
mikroRNA, mioblasty, transkryptom
2028-03-04
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