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Zinc translocation from Zn-sufficient to Zn-deficient roots as an adaptation to heterogeneous Zn availability

Version 1.0

Siemianowski, Oskar; Pypka, Magdalena; Davydenko, Diana; Sowa, Katarzyna; Maksymiuk, Julia; Wróbel, Paweł; Kołodziej, Tomasz; Korecki, Paweł, 2026, "Zinc translocation from Zn-sufficient to Zn-deficient roots as an adaptation to heterogeneous Zn availability", https://doi.org/10.58132/CIQUTM, Dane Badawcze UW, V1

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Description

The deposited data were used in a publication describing a previously unrecognized mechanism in which zinc is translocated between lateral roots, moving from Zn‑sufficient to Zn‑deficient ones, mitigating Zn‑deficiency responses and reducing Zn uptake.

The files are structured according to the figures in the publication https://doi.org/10.1186/s12870-025-07391-z.

Data represent:

Fig. 2 GUS promoter activity analysis in whole roots grown in homogeneous Zn conditions (either with or without added Zn) and in heterogeneous Zn conditions (half of the medium with added Zn and the other half without Zn).

Fig. 3 Zn concentration measurements obtained using atomic absorbance spectroscopy from plant roots grown in homogeneous Zn conditions (either with or without added Zn) and in heterogeneous Zn conditions (half of the medium with added Zn and the other half without Zn).

Fig. 4 and Fig. 5 micro X‑ray fluorescence data from synchrotron studies, including raw data in ZIP archives, normalized PNG/TIFF representations of the raw data, and additional pixel‑intensity analyses such as leaf Zn levels shown in the inset of Fig. 5.

Fig. 6 Zn concentration measurements obtained using atomic absorbance spectroscopy from plant leaves grown in homogeneous Zn conditions (either with or without added Zn) and in heterogeneous Zn conditions (half of the medium with added Zn and the other half without Zn).

Fig. 7 expression analysis of genes involved in Zn transport, including HMA4, ZIP4B, and NAS.

(2026)
Subject
Agricultural Sciences; Earth and Environmental Sciences
Keyword

Zinc transport, Soil heterogeneity, Microelements, Xylem, Phloem, Micro-XRF, Tobacco

Related Publication

Pypka, M., Davydenko, D., Sowa, K. et al. Zinc translocation from Zn-sufficient to Zn-deficient roots as an adaptation to heterogeneous Zn availability. BMC Plant Biol 25, 1341 (2025). https://doi.org/10.1186/s12870-025-07391-z https://doi.org/10.1186/s12870-025-07391-z doi: doi.org/10.1186/s12870-025-07391-z

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fig_4_metadata.odt
Unknown - 12.4 KB - Mar 13, 2026 - 0 Downloads
MD5: 7ef01b0c972565e5bac835d5d2e205a3
License: CC0 Creative Commons Zero 1.0
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fig_5_metadata.odt
Unknown - 12.3 KB - Mar 13, 2026 - 0 Downloads
MD5: c422e5531becaa85b94b8348475f362f
License: CC0 Creative Commons Zero 1.0
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Fig_2_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 3.0 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
25 Variables, 50 Observations - UNF:6:bxaTD97jolCBThdfZEe2Sw==
Data for figure 2 e,f. Quantitative analysis of pNtZIP4B::GUS expression profiles (alpha and beta) in Zn-deficient and Zn-sufficient conditions. Quantification was performed using the apical root segment, defined as the 2 cm region from the root tip. Data presented as a precent of roots representing pNtZIP4B::GUS expression in epidermis, cortex and central cylinder (profile alpha), only in central cylinder (profile beta) and root tips without GUS staining (profile gamma). Data came from 3 independent experiments (biological) with at least 14 plants for each heterogenous treatment at least 9 plants for each homogenous setup. The sample number depends on root penetration into the + Zn/-Zn treatment layers in the heterogeneous setup, and approximate depth matching those layers in the homogeneous setup. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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Fig_3_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 3.6 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
18 Variables, 94 Observations - UNF:6:d01bJ42jFjqJmyxzrkyyUw==
Data for Figure 3. Zn concentration in plants grown in medium with heterogeneous (0/1 µM Zn; 1/0 µM Zn) Zn distribution; homogeneous Zn medium (1/1 µM Zn; 0/0 µM Zn) and in medium from those treatments. The plants were cultivated in two independent experiments. Zn concentration was assessed from at least 7 plants. Transparent soil (medium) was taken from material prepared for experiments and after 14 days. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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Fig_5_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 1.7 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
11 Variables, 24 Observations - UNF:6:aht6WgFJJgRH+i7TKhbOmQ==
Fig_5 inset, analysis of uXRF image (.png) in Fiji using mean pixel intensity across leaf area of plant grown in the following treatments: 1/1, 0/0, 0/2 and 2/0. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium. Missing values encoded as: NA
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Fig_6_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 925 B - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
6 Variables, 43 Observations - UNF:6:G5HWThi7eajY/DZ+Ni3Rkg==
Data for Fig. 6 Zn concentration in N. tobacco shoots of plants grown in Zn heterogeneous (0/2 µM Zn; 2/0 µM Zn) or homogeneous (1/1 µM Zn; 0/0 µM Zn) medium. The plants were cultivated in two independent experiments, six plants per treatment. Zn concentration was assessed from at least 7 plants (from two experiments). These were selected from a group of 12 plants, based on root penetration into the + Zn/-Zn treatment layers in the heterogeneous setup, and approximate depth matching those layers in the homogeneous setup. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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Fig_7_NtHMA_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 14.5 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
10 Variables, 162 Observations - UNF:6:5H/wh4pVAo7xP7IriF0NOQ==
Data for Fig. 7, NtHMA4 expression profiles of NtHMA4, NtNAS and NtZIP4B in roots of N. tobacco grown in Zn heterogeneous (0/2 µM Zn; 2/0 µM Zn) or homogeneous (1/1 µM Zn; 0/0 µM Zn) medium. RNA was isolated from at least nine plants. These were selected from a group of twelve plants, based on root penetration into the + Zn/-Zn treatment layers in the heterogeneous setup, and approximate depth matching those layers in the homogeneous setup. The roots were divided into two (upper and lower) samples before RNA extraction and without pooling. There was no significant differences in expression between upper and lower sample. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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Fig_7_NtNAS_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 14.2 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
10 Variables, 162 Observations - UNF:6:/rzcQZTstEmFhT8VnQ0HhQ==
Data for Fig. 7 NtNAS expression profiles of NtHMA4, NtNAS and NtZIP4B in roots of N. tobacco grown in Zn heterogeneous (0/2 µM Zn; 2/0 µM Zn) or homogeneous (1/1 µM Zn; 0/0 µM Zn) medium. RNA was isolated from at least nine plants. These were selected from a group of twelve plants, based on root penetration into the + Zn/-Zn treatment layers in the heterogeneous setup, and approximate depth matching those layers in the homogeneous setup. The roots were divided into two (upper and lower) samples before RNA extraction and without pooling. There was no significant differences in expression between upper and lower sample. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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Fig_7_NtZIP4B_clean.tab
Tabular Data - Original format: MS Excel (XLSX) - 15.3 KB - Mar 13, 2026 - 0 Downloads
License: CC0 Creative Commons Zero 1.0
10 Variables, 162 Observations - UNF:6:L/OerLSlmvhwGk8Vrm4brg==
Data for Fig. 7 NtZIP4B expression profiles of NtHMA4, NtNAS and NtZIP4B in roots of N. tobacco grown in Zn heterogeneous (0/2 µM Zn; 2/0 µM Zn) or homogeneous (1/1 µM Zn; 0/0 µM Zn) medium. RNA was isolated from at least nine plants. These were selected from a group of twelve plants, based on root penetration into the + Zn/-Zn treatment layers in the heterogeneous setup, and approximate depth matching those layers in the homogeneous setup. The roots were divided into two (upper and lower) samples before RNA extraction and without pooling. There was no significant differences in expression between upper and lower sample. First upper part/second lower part, e.g. 1/0 mean upper medium was 1 uM Zn and lower 0 uM Zn, all other elements as in 1/4 Knop medium Missing values encoded as: NA
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top0_10_middle10_0_bottom_0_10_clean.zip
ZIP Archive - 1.5 GB - Mar 13, 2026 - 0 Downloads
MD5: ff05bf6325745484718f98ba6e66caef
License: CC0 Creative Commons Zero 1.0
Raw uXRF data, details in fig_5.odt
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