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Zimowska-Wypych, Małgorzata, 2025, "The role of TGFβ1 in regulating MMP-9 and MMP-2 activity during myoblast differentiation in vitro", https://doi.org/10.58132/MKB1SZ, Dane Badawcze UW, V1
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Although the role of TGFβ1 in the regulation of ECM protein synthesis is well established, its involvement in the regulation of enzymes, such as MMPs, is still not well understood. In this project, we investigated the relationship between TGFβ1 and MMP-9/MMP-2 in in vitro differentiating myoblasts isolated from rat slow-twitch Soleus or fast-twitch Extensor Digitorum Longus (EDL) muscles. Using siRNA to silence TβR1 expression, suramin as a competitive inhibitor of the TβR1 receptor, and inhibitors of both the canonical and non-canonical TGFβ signaling pathways, we characterized the role of TGFβ1 in regulating MMP-9 and MMP-2 during differentiation of myoblasts derived from slow-twitch and fast-twitch muscles in vitro.
Chociaż rola TGFβ1 w regulacji syntezy białek ECM jest dobrze znana, jego zaangażowanie w regulację enzymów, takich jak MMP, wciąż nie jest w pełni zrozumiane. W projekcie zbadaliśmy zależność między TGFβ1 a MMP-9/MMP-2 w hodowanych in vitro mioblastach, izolowanych z mięśni wolno- (Soleus) lub mięśni szybko-kurczących się (Extensor Digitorum Longus-EDL) szczura. Używając siRNA do wyciszenia ekspresji TβR1, suraminy jako konkurencyjnego inhibitora receptora TβR1 oraz inhibitorów zarówno kanonicznych, jak i niekanonicznych szlaków sygnalizacyjnych TGFβ, scharakteryzowaliśmy rolę TGFβ1 w regulacji MMP-9 i MMP-2 podczas różnicowania in vitro mioblastów izolowanych z mięśni wolno- i szybko-kurczących się.
TGFβ1, MMP-2, MMP-9
Differential Regulation of MMP Activity by TGFβ1 in Fast- and Slow- Twitch Muscle Repair: Insights from EDL and Soleus Muscle-Derived Myoblasts, Kasprzycka P, Ciemerych MA and Zimowska M. Front. Cell Dev. Biol. Sec. Stem Cell Research. Volume 13 - 2025. https://doi.org/10.3389/fcell.2025.1592512 https://doi.org/10.3389/fcell.2025.1592512 doi: 10.3389/fcell.2025.1592512
2028-05-21
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