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Mierzejewski, Bartosz, 2025, "Characterization of CD146+ pericytes isolated from wild-type and mdx mice: proliferation, gene expression profile, differentiation potential, and secretome data", https://doi.org/10.58132/NWQZBI, Dane Badawcze UW, V2
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[ENG:]
This dataset supports a project investigating differences in the proliferative capacity, gene expression profile, protein synthesis, differentiation potential, and secretome composition of CD146-positive (CD146+) pericytes isolated from skeletal muscles of wild-type (WT) and mdx mice, the latter serving as a model for Duchenne muscular dystrophy (DMD).
CD146+ pericytes were isolated from hindlimb skeletal muscles of WT and mdx mice and enriched using magnetic-activated cell sorting (MACS) based on CD146 surface expression. In vitro proliferation was assessed using the CFSE assay. To analyze the gene expression profile, total RNA was extracted from cultured CD146⁺ cells, reverse-transcribed to cDNA, and analyzed by quantitative PCR (qPCR). Protein-level analyses were performed using western blotting to quantify selected markers.
Differentiation potential was evaluated under defined adipogenic, fibrogenic, and myogenic culture conditions. The secretome was analyzed through its functional impact on other cell types using an in vitro angiogenesis assay. Additionally, conditioned medium was collected from CD146⁺ cells to assess the expression of secreted factors.
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[PL:]
Niniejszy zbiór danych stanowi uzupełnienie publikacji dotyczącej analizy transkryptomicznej oraz funkcjonalnej perycytów CD146+ izolowanych z mięśni szkieletowych myszy dzikiego typu (WT) oraz myszy mdx, powszechnie wykorzystywanych jako model dystrofii mięśniowej Duchenne’a (DMD).
Komórki CD146+ wyizolowano z mięśni kończyn tylnych obu linii zwierząt metodą immunoselekcji magnetycznej (MACS) z użyciem przeciwciał anty-CD146. W warunkach in vitro zbadano ich potencjał do proliferacji (test CFSE), profil markerów powierzchniowych (cytometria przepływowa), ekspresję genów (RT-qPCR), aktywność sekrecyjną (test angiogenezy oraz Luminex), a także potencjał różnicowania w kierunku adipogenicznego, fibrogenicznego i miogenicznego. Ponadto wykonano analizę ekspresji białek wybranych markerów metodą western blot.
Poniższy zbiór danych zawiera
Konkretnie:
duchenne muscular dystrophy, pericyte, differentiation, fibrosis
Mierzejewski B, Grabowska I, Jackowski D, Irhashava A, Michalska Z, Stremińska W, Jańczyk-Ilach K, Ciemerych MA, Brzoska E. Mouse CD146+ muscle interstitial progenitor cells differ from satellite cells and present myogenic potential. Stem Cell Res Ther. 2020 Aug 6;11(1):341. https://doi.org/10.1186/s13287-020-01827-z https://doi.org/10.1186/s13287-020-01827-z doi: 10.1186/s13287-020-01827-z
CC BY - Creative Commons Attribution 4.0
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