Wydział Biologii

Dataset description:

Rows: Individual nectar samples

Columns:

1. sample — Unique identifier of the nectar sample (string; e.g. G4O).
2. species — Plant species name (string; binomial, underscores instead of spaces; e.g. Impatiens_walleriana).
3. f__… — Columns prefixed with f__ represent fungal families (e.g. f__Aspergillaceae, f__Bartaliniaceae). Each contains integer read counts of ITS amplicon sequencing reads assigned to that family.

• Example: f__Aspergillaceae — read counts for family Aspergillaceae.
• Zero means no reads assigned.

Pollinator visitation columns:

• bumblebee — Number of visits by bumblebees recorded for the given sample (integer).
• fly — Number of visits by non-hoverfly Diptera (integer).
• ant — Number of visits by ants (integer).
• hoverfly — Number of visits by Syrphidae (hoverflies) (integer).
• hymenoptera — Number of visits by non-bee Hymenoptera (integer).
• other — Number of visits by other insect groups (integer).

Diversity indices (calculated from fungal community data):

• richness — Observed richness (number of fungal OTUs detected).
• sha_div — Shannon diversity index.
• invsimp — Inverse Simpson diversity index.
• chao — Chao1 richness estimator.

Methods of obtaining statistical results:

All analyses were performed in R (Team, 2024). To assess the alpha diversity of nectar-associated microbial communities, two complementary indices were calculated: Shannon diversity and the Chao1 richness estimator. Shannon diversity was used as the primary metric for statistical analysis and visualization, as it reflects both species richness and evenness, providing a comprehensive measure of community complexity. Chao1 was included to estimate total richness, including rare or undetected taxa, which is particularly relevant in systems with uneven sequencing depth or low detection rates. Both indices were calculated from taxon abundance tables derived from ITS amplicon sequencing using the vegan (VEGAN, 2003) and phyloseq packages (McMurdie and Holmes, 2013).