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The role of CD146+ pericytes in skeletal muscle regeneration in vivo

Version 1.0

Mierzejewski, Bartosz, 2026, "The role of CD146+ pericytes in skeletal muscle regeneration in vivo", https://doi.org/10.58132/UC0SJ5, Dane Badawcze UW, V1

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Description

This dataset supports a project investigating the role of the CD146+ cell population in skeletal muscle development and regeneration, as well as the impact of exogenous CD146+ cell engraftment on vascularization, fibrosis, and adipose tissue deposition in dystrophic skeletal muscle of mdx mice.

CD146+ pericytes were isolated from hindlimb skeletal muscles of GFP-expressing mice and enriched using magnetic-activated cell sorting (MACS) based on CD146 surface expression. The cells were injected into skeletal muscles of mdx mice subjected to either BaCl₂-induced injury or treadmill-induced damage. The role of CD146⁺ cells during skeletal muscle development and regeneration was evaluated using histological staining and immunohistochemistry on tissue cryosections. RNA was extracted from either WT or mdx mouse skeletal muscles during regeneration, and qPCR was performed to monitor changes in gene expression levels.

The impact of CD146+ cell engraftment was evaluated by quantifying fibers with centrally located nuclei (an indicator of regeneration), measuring muscle fiber cross-sectional area, and performing histological staining, immunohistochemistry, and qPCR analyses.

This dataset includes:

  • .xlsx (some with .ods copies) and .txt files

Specifically:

1.    CSA_BaCl2.xlsx - Excel file containing raw measurements of fiber size

2.    2way_ANOVA_CSA_BaCl2_freaquency.txt - text file (.txt) showing statistical analysis (two-way ANOVA) of the fiber size distribution

3.    central_nuclei_ BaCl2.xlsx - Excel file containing raw measurements of centrally nucleated fibers

4.    CSA_treadmill.xlsx- Excel file containing raw measurements of fiber size

5.    2way_ANOVA_CSA_treadmil_frequency.txt - text file (.txt) showing statistical analysis (two-way ANOVA) of the fiber size distribution

6.    central_nuclei_ treadmill.xlsx- Excel file containing raw measurements of centrally nucleated fibers

7.    Muscle_mass_BaCl.xlsx- Excel file containing raw measurements of muscle mass

8.    Ordinary_one-way_ANOVA_of_muscle_mass_BaCl.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the muscle mass for BaCl injured muscles

9.    Muscle_mass_treadmill.xlsx- Excel file containing raw measurements of muscle mass

10. Ordinary_one-way_ANOVA_of_muscle_mass_treadmill.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of the muscle mass for treadmill injured muscles

11. Blood_vessels_area_BaCl_raw_data.xlsx- Excel file containing raw measurements of blood vessels area in BaCl injured muscles

12. Ordinary_one-way_ANOVA_of_vessels_BaCl.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the blood vessels area for BaCl injured muscles

13. Blood_vessels_area_treadmill_raw_data.xlsx - Excel file containing raw measurements of blood vessels area in treadmill injured muscles

14. Ordinary_one-way_ANOVA_of_vessels_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the blood vessels area for treadmill injured muscles

15. Perilipin_raw_data.xlsx - Excel file containing raw measurements of perilipin expressing cells

16. Ordinary_one-way_ANOVA_of_perilipin_BaCl.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of the perilipin expressing cells in BaCl injured muscles

17. Ordinary_one-way_ANOVA_of_perilipin_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of the perilipin expressing cells in treadmill injured muscles

18. Masson_Trichrome_collagen_area_BaCl_raw_data.xlsx- Excel file containing raw measurements of collagen area in BaCl injured muscles

19. Ordinary_one-way_ANOVA_of_collagen _area_AVE_BaCl.txt- text file (.txt) showing statistical analysis (one-way ANOVA) of of collagen area in in BaCl injured muscles

20. Masson_Trichrome_collagen_area_treadmill_raw_data.xlsx- Excel file containing raw measurements of collagen area in treadmill injured muscles

21. Unpaired_t_test_of_collagen_area_treadmill.txt - text file (.txt) showing statistical analysis (one-way ANOVA) of of collagen area in in treadmill injured muscles

22. MCAM_WT_fluorescence_area_raw_data.xlsx - Excel file containing raw measurements of MCAM expressing cells area in WT muscles

23. MCAM_WT_fluorescence_area_t_test.txt - text file (.txt) showing statistical analysis (t-test) of of collagen area in WT muscles

24. MCAM_MDX_fluorescence_area.xlsx- Excel file containing raw measurements of MCAM expressing cells area in MDX muscles

25. MCAM_MDX_fluorescence_area_ANOVA.txt - text file (.txt) showing statistical analysis (t-test) of of collagen area in MDX muscles

26. qPCR_DMD_marker_expression_day_DMD_9_12.xlsx – Excel file containing raw Cq data for gene expression in MDX mouse muscles

27. qPCR_DMD_marker_expression_day_DMD_5_8.xlsx - Excel file containing raw Cq data for gene expression in MDX mouse muscles

28. qPCR_DMD_marker_expression_day_DMD_1_4.xlsx - Excel file containing raw Cq data for gene expression in MDX mouse muscles

29. qPCR_WT_marker_expression_day_14.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles

30. qPCR_WT_marker_expression_day_7.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles

31. qPCR_WT_marker_expression_day_3.xlsx - Excel file containing raw Cq data for gene expression in WTmouse muscles

32. qPCR_WT_marker_expression_control.xlsx - Excel file containing raw Cq data for gene expression in WT mouse muscles

33. treadmill_part1_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

34. treadmill_part2_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

35. treadmill_part3_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

36. treadmill_part4_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

37. treadmill_part5_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

38. treadmill_part6_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in treadmill injured mdx mouse muscles

39. BaCl2_part1_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles

40. BaCl2_part2_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles

41. BaCl2_part3_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles

42. BaCl2_part4_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles

43. BaCl2_part5_Abs Quant.txt - text file (.txt) containing raw Cq data for gene expression in BaCl injured mdx mouse muscles

(2026-03-12)
Subject
Medicine, Health and Life Sciences
Keyword

duchenne muscular dystrophy, pericyte, fibrosis, angiogenesis, pathology

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CSA_BaCl2.tab
Tabular Data - Original format: MS Excel (XLSX) - 1.4 KB - Mar 17, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
7 Variables, 31 Observations - UNF:6:qWPTsoCXvVmm9P2ZcW4LrQ==
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2way_ANOVA_of_CSA_BaCl2_frequency.txt
Plain Text - 2.4 KB - Mar 17, 2026 - 0 Downloads
MD5: c32c16a6e77b614e57debbf9c6cc51b3
License: CC BY - Creative Commons Attribution 4.0
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CSA_treadmill.tab
Tabular Data - Original format: MS Excel (XLSX) - 1.8 KB - Mar 17, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
10 Variables, 31 Observations - UNF:6:6gPB1Qgck6IOmcxqhe8g4w==
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2way_ANOVA_CSA_treadmilll_frequency.txt
Plain Text - 2.4 KB - Mar 17, 2026 - 0 Downloads
MD5: b21c608852fab2f916b7bc356af176ee
License: CC BY - Creative Commons Attribution 4.0
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muscle_mass_BaCl.tab
Tabular Data - Original format: MS Excel (XLSX) - 212 B - Mar 17, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
2 Variables, 10 Observations - UNF:6:L48/WYZoTewiVbGhJRK73w==
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Ordinary_one_way_ANOVA_of_muscle_mass_BaCl.txt
Plain Text - 826 B - Mar 17, 2026 - 0 Downloads
MD5: 8eb762a2edb4ac3b24c0b42284e007eb
License: CC BY - Creative Commons Attribution 4.0
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muscle_mass_treadmill.tab
Tabular Data - Original format: MS Excel (XLSX) - 186 B - Mar 17, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
2 Variables, 9 Observations - UNF:6:n/Y6s3Gwe9gb5LvM/f+1rQ==
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Ordinary_one_way_ANOVA_of_muscle_mass_treadmill.txt
Plain Text - 803 B - Mar 17, 2026 - 0 Downloads
MD5: 78ac0b766a13cafce9d946e5fdf31f6b
License: CC BY - Creative Commons Attribution 4.0
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blood_vessles_area_BaCl2_raw_data.tab
Tabular Data - Original format: MS Excel (XLSX) - 2.5 KB - Mar 17, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
5 Variables, 99 Observations - UNF:6:W2TrkxSyMV4YtcuiZvi92g==
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blood_vessles_area_BaCl2_raw_data.ods
Unknown - 39.0 KB - Mar 17, 2026 - 0 Downloads
MD5: 24ee45c0572cc025822f38068e8b9b32
License: CC BY - Creative Commons Attribution 4.0
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