Metrics
0 Downloads
Share Dataset

Share this dataset on your favorite social media networks.

Light-induced changes of thylakoid grana structure

Version 1.0

Mazur, Radosław; Wójtowicz, Joanna; Jakubauskas, Dainius; Sokolova, Anna; Garvey, Christopher; Mortensen, Kell; Jensen, Poul Erik; Kirkensgaard, Jacob JK; Kowalewska, Łucja, 2025, "Light-induced changes of thylakoid grana structure", https://doi.org/10.58132/UZI5C7, Dane Badawcze UW, V1

Learn about Data Citation Standards.

Description

This dataset encompasses comprehensive analyses of thylakoid membrane structural dynamics in response to light exposure. The study investigates temporal changes in thylakoid network organization, particularly focusing on grana structural modifications during dark-to-light transitions.

File name: CLSM_numerical_data.csv – volumetric data and thickness of the thylakoid network from dark and light conditions of Arabidopsis and Ficus plants grown in greenhouse conditions. V denotes volume in µm3 units; A denotes area in µm2 units; T denotes thylakoid thickness in µm units; sign "-" denotes lack of the data.

File name: TEM_numerical_data.csv – TEM structural parameters of grana thylakoids of Arabidopsis and Ficus from dark and light conditions. SRD denotes stacking repeat distance in nanometer units. LF denotes percentage (%) of lumen fraction in SRD change. GD denotes grana diameter, GH denotes grana height both in nanometers units; sign "-" denotes lack of the data.

File name: immunoblot_densitometry.csv – densitometric analysis of immunodetection of Lhcb5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB in thylakoid samples of Arabidopsis and Ficus from dark and light (30, 50, and 90 min) conditions. Column A – gel lane No; column B – sample description; columns C-AF – pixel intensity data.

File name: immunoblots.zip – compressed 31 files containing 30 files in tiff or jpg format and 1 readme.txt file. The Images represent immunoblots of thylakoid proteins: Lhbc5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB for Arabidopsis and Ficus plants from dark conditions (0 min) or exposed to light (30, 50, and 90 min). The sample loading from left to right: Arabidopsis 0 min, Ficus 0 min, Arabidopsis 30 min, Ficus 30 min, Arabidopsis 50 min, Ficus 50 min, Arabidopsis 90 min, Ficus 90 min.

File name: 77_K_fluorescence_spectra.csv – raw data for chlorophyll emission spectra of Arabidopsis and Ficus thylakoids isolated from dark conditions.

File name: P515_single_light_intensity_data.csv – raw data of P515 signal (recorded on Dual-PAM device with P515 module) at single actinic light intensity (500 µmol of photons m-2 s-1) for dark-adapted Arabidopsis and Ficus plants.

File name: P515_light_curve_data.csv – raw data of P515 signal (recorded on Dual-PAM device with P515 module) at increasing actinic light intensities 15, 99, 309, 773, 1764, and 3963 µmol of photons m-2 s-1 for dark-adapted Arabidopsis and Ficus plants.

Subject
Medicine, Health and Life Sciences
Keyword

thylakoid membrane, transmission electron microscopy, light response, grana, Arabidopsis thaliana, Ficus sp

CC BY - Creative Commons Attribution 4.0 CC BY - Creative Commons Attribution 4.0

Find
1 to 8 of 8 Files
immunoblots.zip
ZIP Archive - 10.1 MB - Apr 15, 2025 - 0 Downloads
MD5: cb84ce2d918ce2261d9a93812fedb6f7
License: CC BY - Creative Commons Attribution 4.0
compressed 31 files containing 30 files in tiff or jpg format and 1 readme.txt file. The Images represent immunoblots of thylakoid proteins: Lhbc5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB for Arabidopsis and Ficus plants from dark conditions (0 min) or exposed to light (30, 50, and 90 min). The sample loading from left to right: Arabidopsis 0 min, Ficus 0 min, Arabidopsis 30 min, Ficus 30 min, Arabidopsis 50 min, Ficus 50 min, Arabidopsis 90 min, Ficus 90 min.
Download
immunoblot_densitometry.tab
Tabular Data - Original format: Comma Separated Values - 2.0 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
32 Variables, 8 Observations - UNF:6:qr6fXlhu0wArVQlg6XojYA==
Immunoblot densitometry.csv – densitometric analysis of immunodetection of Lhcb5, Lhcb4, Lhcb2, Lhcb2-P, D1, D1-P, Lhca1, AtpB, PsaB in thylakoid samples of Arabidopsis and Ficus from dark and light (30, 50, and 90 min) conditions. Column A – gel lane No; column B – sample description; columns C-AF – pixel intensity data.
Preview
77K_fluorescence_spectra.tab
Tabular Data - Original format: Comma Separated Values - 12.2 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
10 Variables, 201 Observations - UNF:6:wAh4XIJIHoOmaLVBFLPNGg==
: 77 K fluorescence spectra.csv – raw data for chlorophyll emission spectra of Arabidopsis and Ficus thylakoids isolated from dark conditions
Preview
PAM_fluorescence.tab
Tabular Data - Original format: Comma Separated Values - 1.5 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
11 Variables, 25 Observations - UNF:6:sunj6spwBXHaHzaeG0pO5w==
PAM fluorescence.csv - raw data (recorded on Dual-PAM device) of Y(I) and Y(II) parameters for dark-adapted Arabidopsis plants.
Preview
TEM_numerical_data.tab
Tabular Data - Original format: Comma Separated Values - 26.5 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
38 Variables, 113 Observations - UNF:6:GTzX+nbKOK3gDZmQbEXZ1w==
TEM numerical data.csv – TEM structural parameters of grana thylakoids of Arabidopsis and Ficus from dark and light conditions. SRD denotes stacking repeat distance in nanometer units. LF denotes percentage (%) of lumen fraction in SRD change. GD denotes grana diameter, GH denotes grana height both in nanometers units; sign "-" denotes lack of the data
Preview
CLSM_numerical_data.tab
Tabular Data - Original format: Comma Separated Values - 8.2 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
24 Variables, 66 Observations - UNF:6:oMYuKVKRz8AC/7CI8qTVLA==
CLSM numerical data.csv – volumetric data and thickness of the thylakoid network from dark and light conditions of Arabidopsis and Ficus plants grown in greenhouse conditions. V denotes volume in µm3 units; A denotes area in µm2 units; T denotes thylakoid thickness in µm units; sign "-" denotes lack of the data
Preview
P515_single_light_intensity_data.tab
Tabular Data - Original format: Comma Separated Values - 266.6 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
16 Variables, 2458 Observations - UNF:6:vsVvCDcmkHuLgPQA5xv2kQ==
Raw data of P515 signal (recorded on Dual-PAM device with P515 module) at single actinic light intensity (500 µmol of photons m-2 s-1) for dark-adapted Arabidopsis and Ficus plants.
Preview
P515_light_curve_data.tab
Tabular Data - Original format: Comma Separated Values - 237.6 KB - Apr 15, 2025 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
17 Variables, 2056 Observations - UNF:6:RJqAXZ4pQUjmSIAC/qP5rw==
Raw data of P515 signal (recorded on Dual-PAM device with P515 module) at increasing actinic light intensities 15, 99, 309, 773, 1764, and 3963 µmol of photons m-2 s-1 for dark-adapted Arabidopsis and Ficus plants.
Preview
Select File(s)

Please select a file or files to be deleted.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select a file or files to be edited.

Select File(s)

Please select a file or files to be edited.

Select File(s)

Please select a file or files to be edited.

Edit license

For selected file(s) set a license to

Select File(s)

Please select a file or files to be downloaded.

Select File(s)

Please select a file or files for access request.

Select File(s)

Please select restricted file(s) to be unrestricted.

Request Access

  You need to Log In/Sign Up to request access to this file.

Continue
Dataset Terms

Please confirm and/or complete the information needed below in order to continue.

Asterisks indicate required fields

Request Access

Access to file(s) subject to additional consent under following conditions:

Accept
Package File Download
Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Click Continue to download the files you have access to download.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Embargo

Embargo

Are you sure you want to lift the embargo?

Once you lift the embargo, you will not be able to set it again.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Dataset Private URL

Use a Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.

Private URL has not been created.

Dataset Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.

Submit for Review

You will not be able to make changes to this dataset while it is in review.

Submit
Publish Dataset

This dataset cannot be published until Wydział Biologii is published. Would you like to publish both right now?

Once you publish this dataset it must remain published.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until Wydział Biologii is published by its administrator.

Publish Dataset

This dataset cannot be published until Wydział Biologii and Dane Badawcze UW are published.

Return for modification


Send a copy of this message to the return e-mail address.
OK
Deaccession Dataset

Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.